L-argininamide improves the refolding more effectively than L-arginine.

L - Argininamide improves the refolding more effectively than 1 L - arginine 2 3

Optimizing refolding condition for recombinant tissue plasminogen activator

Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...

Effect of L-Arginine Modified Magnetic Nanoparticles (RMNPs) on Lysozyme in the Presence or Absence of Urea

L-Arginine (Arg or R) is a non-toxigenic, metabolically versatile and conditionally essential amino acid. Single-pot synthesis of Arg modified magnetic nanoparticles (RMNPs) was achieved using magnetite nanoparticles (MNPs) and binary function of Arg as a functional group and an alkali precipitator. This modification is supposed to offer several advantages to the MNPs from a bio-application vie...

Optimizing Primary Recovery and Refolding of Human Interferon-b from Escherichia coli Inclusion Bodies

Background: The refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. Objectives: The purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produ...

Synergistic Protection of L-Arginine and Vitamin E On Lipid Peroxidation of Asthenospermic Patients

Background Lipid peroxidation is known to cause various impairments to sperm cells and may play a major role in the etiology of male infertility. Asthenospermia is the main factor of male infertility and has significantly higher level of peroxidation than in normozoospermic males. MaterialsAndMethods Using thiobarbituric acid (TBA) assay procedure, we have determined the level of lipid peroxida...